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Invitrogen
PPAR alpha Monoclonal Antibody (3B6/PPAR)
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FIGURE: 1 / 19
PPAR alpha Antibody (MA1-822) in WB
Western blot analysis of PPAR alpha was performed by loading 25 µg of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with a PPAR alpha monoclonal antibody (Product # MA1-822) at a dilution of 1:1000 (Hela and Jurkat) and 1:500 (NIH-3T3) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr a... View More
Product Details
MA1-822
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
C. elegans,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3B6/PPAR
Immunogen
Purified recombinant PPAR alpha protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues.
MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates.
The MA1-822 immunogen is purified recombinant PPAR alpha protein.
Target Information
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha
Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha
UniProt ID: (Human) Q07869, (Rat) P37230, (Mouse) P23204
Entrez Gene ID: (Human) 5465, (Rat) 25747, (Mouse) 19013
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II repressing transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
drug binding
zinc ion binding
lipid binding
phosphatase binding
protein domain specific binding
ubiquitin conjugating enzyme binding
protein complex binding
sequence-specific DNA binding
NFAT protein binding
MDM2/MDM4 family protein binding
receptor activity
metal ion binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
transcription initiation from RNA polymerase II promoter
lipid metabolic process
fatty acid metabolic process
heart development
epidermis development
negative regulation of macrophage derived foam cell differentiation
negative regulation of receptor biosynthetic process
negative regulation of cholesterol storage
negative regulation of sequestering of triglyceride
fatty acid transport
intracellular receptor signaling pathway
positive regulation of fatty acid beta-oxidation
negative regulation of protein binding
negative regulation of appetite
response to insulin
circadian regulation of gene expression
behavioral response to nicotine
wound healing
lipoprotein metabolic process
regulation of circadian rhythm
steroid hormone mediated signaling pathway
cellular lipid metabolic process
positive regulation of gluconeogenesis
negative regulation of blood pressure
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fatty acid oxidation
negative regulation of inflammatory response
enamel mineralization
regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter
regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter
regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter
negative regulation of neuron death
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of leukocyte cell-cell adhesion
negative regulation of transcription regulatory region DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of gene expression
regulation of fatty acid metabolic process
glucose metabolic process
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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