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Invitrogen
eGFP Monoclonal Antibody (F56-6A1.2.3)
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FIGURE: 1 / 7
eGFP Antibody (MA1-952) in ICC/IF
Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with eGFP Monoclonal Antibody (F56-6A1.2.3) (Product # MA1-952) at 1:100 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mous... View More
Product Details
MA1-952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
C. elegans,
Tag
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
F56-6A1.2.3
Immunogen
Full length enhanced green fluorescent protein (eGFP)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-952 contains 100 µg of in vitro produced, Protein A purified IgG (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA1-952 detects eGFP and eGFP tagged proteins.
MA1-952 has been successfully used in Western blot and immunofluorescence applications.
The MA1-952 immunogen is full length enhanced green fluorescent protein (eGFP).
Target Information
eGFP (green fluorescent protein) fluorescent protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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