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Invitrogen
Cytokeratin Pan Type I Monoclonal Antibody (AE1)
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FIGURE: 1 / 9
Cytokeratin Pan Type I Antibody (MA5-13144) in ICC/IF
Immunofluorescence analysis of Cytokeratin Low Molecular Weight was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8/18 Monoclonal Antibody (Product # MA5-13144) at 5µg/mL in 0.1% BSA and incub... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-13144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
Human,
Insect,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AE1
Immunogen
Human epidermal keratin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13144 targets Cytokeratin Low Molecular Weight in WB, IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples. This antibody is not recommended for NIH-3T3 cells in immunofluorescent applications.
The MA5-13144 immunogen is human epidermal keratin.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; Acidic Cytokeratin; alpha keratin; cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; cytokeratin endo B; cytokeratin VIB; cytokeratin VII; cytokeratin-10; cytokeratin-14; cytokeratin-15; cytokeratin-16; cytokeratin-18; cytokeratin-19; cytokeratin-6B; cytokeratin-7; cytokeratin-9; epidermal keratin 10; epidermal keratin type I; epidermal keratin VII; epidermolytic hyperkeratosis; keratosis palmaris et plantaris; focal non-epidermolytic palmoplantar keratoderma; HMWCK; intermediate filament; intermediate filament protein; intermediate filament protein K16; K10; K14; K15; K18; K19; K9; keratin; keratin 10; keratin 10 (epidermolytic hyperkeratosis); keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 14-like; keratin 15; keratin 15, type I; keratin 16 homolog; keratin 16, type I; keratin 18, type I; keratin 19; keratin 19, type I; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; keratin, type I cytoskeletal 59 kDa; keratin, type I, 40-kd; keratin-10; keratin-15, basic; keratin-15, beta; ORF; pan keratin; pankeratin; PIG46; spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytokeratin; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; type I keratin Ka14; type I keratin KA15; type I keratin KA16; type I keratin KA19; unnamed protein product
Gene Aliases: BCIE; BIE; CK-14; CK-16; CK-18; CK-19; CK-9; CK10; CK14; CK15; CK16; CK18; CK19; CYK18; D130054E02Rik; EBS1; EBS1A; EBS1B; EBS1C; EBS1D; EBS3; EBS4; EHK; EHK2; EHK2A; EHK2B; EndoC; EPPK; FNEPPK; GK-19; IHL; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K9; Ka10; Ka14; Ka15; Ka16; Ka19; keratin-19; KPP; Krt-1.10; Krt-1.14; Krt-1.19; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; Krt1-9; Krt14; Krt14l; KRT15; KRT16A; KRT19; KRT9L; NEPPK; NFJ; PC1
UniProt ID: (Bovine) P06394, (Human) P13645, (Human) P02533, (Chicken) Q6PVZ1, (Human) P19012, (Human) P08779, (Human) P05783, (Bovine) P08728, (Human) P08727, (Chicken) O93256, (Human) P35527, (Mouse) P02535, (Rat) Q6IFW6, (Mouse) Q61781, (Rat) Q6IFV1, (Mouse) Q61414, (Rat) Q6IFV3, (Mouse) Q9Z2K1, (Rat) Q5BJY9, (Mouse) P05784, (Mouse) P19001, (Rat) Q63279, (Mouse) Q6RHW0, (Rat) Q8CIS9
Entrez Gene ID: (Bovine) 281888, (Chicken) 771977, (Rabbit) 100009029, (Human) 3858, (Chicken) 408040, (Human) 3861, (Bovine) 404111, (Chicken) 408039, (Rabbit) 100341120, (Bovine) 507464, (Human) 3866, (Human) 3868, (Rabbit) 100341376, (Rabbit) 100354174, (Chicken) 101749333, (Bovine) 506480, (Human) 3875, (Bovine) 514812, (Human) 3880, (Chicken) 395861, (Chicken) 420041, (Human) 3857, (Mouse) 16661, (Rat) 450225, (Mouse) 16664, (Rat) 287701, (Mouse) 16665, (Rat) 287700, (Mouse) 16666, (Rat) 303530, (Rat) 294853, (Mouse) 16668, (Mouse) 16669, (Rat) 360626, (Mouse) 107656, (Rat) 266717
structural molecule activity
protein heterodimerization activity
protein binding
structural constituent of epidermis
structural constituent of cytoskeleton
keratin filament binding
scaffold protein binding
RNA binding
structural constituent of muscle
cadherin binding involved in cell-cell adhesion
macromolecular complex binding
cytoskeletal protein binding
Notch signaling pathway
response to estrogen
sarcomere organization
cell differentiation involved in embryonic placenta development
morphogenesis of an epithelium
epithelial cell differentiation
wound healing
intermediate filament organization
negative regulation of keratinocyte differentiation
keratinocyte development
epidermis development
keratinocyte differentiation
positive regulation of epidermis development
protein heterotetramerization
cornification
cellular response to calcium ion
spermatogenesis
skin development
inflammatory response
cytoskeleton organization
negative regulation of cell migration
keratinization
hair cycle
innate immune response
keratinocyte migration
establishment of skin barrier
response to fructose
response to nutrient levels
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
hepatocyte differentiation
mesenchymal stem cell differentiation
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cholangiocyte apoptotic process
intermediate filament bundle assembly
positive regulation of keratinocyte differentiation
response to radiation
response to zinc ion
response to ionizing radiation
negative regulation of epithelial cell differentiation
biological_process
anatomical structure morphogenesis
cell-cell adhesion
intermediate filament-based process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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