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Invitrogen
PSA Monoclonal Antibody (EP1588Y)
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图: 1 / 4
PSA Antibody (MA5-14470) in ICC/IF
Immunofluorescent analysis of Prostate Specific Antigen (green) showing staining in the cytoplasm of PC-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Prostate Specific Antigen monoclonal antibody (Product # MA5-14470) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with ... View More
产品信息
MA5-14470
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
EP1588Y
抗原
A synthetic peptide corresponding to residues near the C-terminus of human PSA
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
MA5-14470 targets Prostate Specific Antigen in IHC (P), ICC/IF and WB applications and shows reactivity with Human samples, however this antibody is not recommended for human colon and mouse prostate tissue sections. This antibody does not react with rat tissue in Western blot applications.
The MA5-14470 immunogen is a synthetic peptide corresponding to residues near the C-terminus of human PSA.
靶标信息
PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and is abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. PSA is used to confirm prostatic acinar cell origin in primary and metastatic carcinoma and to rule out non-prostatic carcinoma mimics.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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