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Invitrogen
PARP1 Monoclonal Antibody (C.384.8)
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图: 1 / 10
PARP1 Antibody (MA5-15031) in ICC/IF
Immunofluorescent analysis of PARP (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a PARP monoclonal antibody (Product # MA5-15031) at a dilution of 1:200 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1hr at room temperature. Nuclei (blue) were ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-15031
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
C.384.8
抗原
Synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
438 µg/mL
规格
43.8 µg
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with PARP-2 and PARP-3.
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase PARP-1; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1
基因别名: 5830444G22Rik; Adprp; ADPRT; Adprt1; AI893648; ARTD1; C80510; PARP; Parp-1; PARP1; PPOL; sPARP-1
UniProt ID: (Rat) P27008
Entrez Gene ID: (Rat) 25591, (Mouse) 11545
DNA binding
NAD+ ADP-ribosyltransferase activity
transcription factor binding
zinc ion binding
transferase activity, transferring pentosyl groups
enzyme binding
protein kinase binding
identical protein binding
poly(A) RNA binding
protein N-terminus binding
NAD binding
R-SMAD binding
chromatin binding
protein binding
transferase activity
transferase activity, transferring glycosyl groups
metal ion binding
DNA metabolism protein
telomere maintenance
DNA metabolic process
base-excision repair
double-strand break repair
transcription, DNA-templated
protein ADP-ribosylation
transforming growth factor beta receptor signaling pathway
protein autoprocessing
signal transduction involved in regulation of gene expression
cellular response to insulin stimulus
regulation of growth rate
DNA damage response, detection of DNA damage
positive regulation of transcription from RNA polymerase II promoter
positive regulation of SMAD protein import into nucleus
protein poly-ADP-ribosylation
cellular response to superoxide
positive regulation of transcription regulatory region DNA binding
DNA repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
mitochondrion organization
mitochondrial DNA metabolic process
cellular response to oxidative stress
protein modification process
mitochondrial DNA repair
behavioral response to cocaine
regulation of single strand break repair
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
