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          • Primary Antibodies ›
          • MYL9 Antibodies

          Invitrogen

          Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1)

          7 References
          View all (17) MYL9 antibodies

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          Cite Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1)

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          • Antibody Testing Data (3)
          Phospho-MYL9 (Ser19) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-MYL9 (Ser19) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-MYL9 (Ser19) Antibody (MA5-15163) in ICC/IF

          Immunofluorescent analysis of Phospho-Myosin Light Chain 2 pSer19 in HeLa cells using a Phospho-Myosin Light Chain 2 pSer19 onoclonal antibody (Product # MA5-15163) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-MYL9 (Ser19) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-MYL9 (Ser19) Antibody in Western Blot (WB)
          Phospho-MYL9 (Ser19) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1)

          Product Details

          MA5-15163

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:100-1:200
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Pig, Rat

          Published species

          Human, Mouse, Rat, Xenopus

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          R.179.1

          Immunogen

          Synthetic phosphopeptide corresponding to residues surrounding pSer19 of human myosine light chain 2 (smooth muscle)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          890 µg/mL

          Amount

          89 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10979087

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with the cardiac isoform of myosin light chain 2.

          Target Information

          Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin light chain may regulate muscle contraction by modulating the ATPase activity of myosin heads. It also binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 20 kDa myosin light chain; epididymis secretory sperm binding protein; MGC3505; MYL9; myosin light chain, regulatory C; myosin regulatory light chain; myosin regulatory light chain 1; myosin regulatory light chain 2, smooth muscle isoform; myosin regulatory light chain 9; myosin regulatory light chain MRLC1; myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; unnamed protein product

          View more View less

          Gene Aliases: LC20; MLC-2C; MLC2; MLC20; MMIHS4; MRLC1; Mylc2c; MYRL2; RLC-C

          View more View less

          UniProt ID: (Pig) P29269, (Human) P24844, (Rat) Q64122, (Mouse) Q9CQ19

          View more View less

          Entrez Gene ID: (Pig) 100157760, (Human) 10398, (Bovine) 505964, (Rat) 296313, (Mouse) 98932

          View more View less

          Function(s)
          calcium ion binding structural constituent of cytoskeleton protein binding structural constituent of muscle myosin heavy chain binding actin or actin-binding cytoskeletal protein
          Process(es)
          regulation of muscle contraction vascular smooth muscle contraction myofibril assembly stress fiber assembly platelet aggregation
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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