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Invitrogen
RICTOR Monoclonal Antibody (7B3)
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FIGURE: 1 / 6
RICTOR Antibody (MA5-15681) in ICC/IF
Immunofluorescent analysis of Rictor (magenta) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a Rictor monoclonal antibody (Product # MA5-15681) at a dilution of 1:200 in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-mouse IgG Secondary Antibody, Alexa Fluor 647 conjugate (Product # A32728) at a dilution of 1:1000 for 1 hou... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7B3
Immunogen
Purified recombinant fragment of human RICTOR expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15681 targets RICTOR in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The MA5-15681 immunogen is purified recombinant fragment of human RICTOR expressed in E. Coli.
MA5-15681 detects RICTOR which has a predicted molecular weight of approximately 192kDa.
Target Information
RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AVO3 homolog; DKFZp686B11164; mAVO3; MGC39830; pianissimo; protein pianissimo; TORC2-specific protein AVO3; unnamed protein product
Gene Aliases: 4921505C17Rik; 6030405M08Rik; AVO3; D530039E11Rik; hAVO3; PIA
UniProt ID: (Human) Q6R327, (Mouse) Q6QI06
Entrez Gene ID: (Human) 253260, (Mouse) 78757
positive regulation of endothelial cell proliferation
cytoskeleton organization
lipid biosynthetic process
embryo development ending in birth or egg hatching
actin cytoskeleton organization
positive regulation of cell growth
positive regulation of cell migration
cellular response to nutrient levels
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
TORC2 signaling
negative regulation of apoptotic process
regulation of protein kinase B signaling
positive regulation of protein kinase B signaling
regulation of protein phosphorylation
regulation of gene expression
positive regulation of actin filament polymerization
regulation of peptidyl-serine phosphorylation
regulation of phosphorylation
protein kinase B signaling
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of establishment of cell polarity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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