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Invitrogen
USP5 Monoclonal Antibody (OTI2E3)
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FIGURE: 1 / 8
USP5 Antibody (MA5-25140) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a USP5 monoclonal antibody (Product # MA5-25140) at a dilution of 1:50.
Product Details
MA5-25140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2E3
Immunogen
Full length human recombinant protein of USP5 produced in HEK293T cell
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.45 mg/mL
Amount
45 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5
Gene Aliases: ISOT; USP5
UniProt ID: (Human) P45974
Entrez Gene ID: (Human) 8078, (Dog) 486718, (Rat) 297593
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
zinc ion binding
hydrolase activity
ubiquitin binding
metal ion binding
ubiquitinyl hydrolase activity
omega peptidase activity
cysteine protease
negative regulation of T cell mediated immune response to tumor cell
proteolysis
regulation of mitotic cell cycle
protein ubiquitination
protein deubiquitination
regulation of protein stability
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of translation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of T cell activation
protein K48-linked deubiquitination
regulation protein catabolic process at presynapse
positive regulation of proteasomal protein catabolic process
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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