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RAB7A Antibody (MA5-32340) in ICC/IF
Immunofluorescence of RAB7A was performed using HeLa WT and RAB7A KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stained ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-32340
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SN202-03
Immunogen
Synthetic peptide within Human RAB7 aa 158-207.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.06 mg/mL
Amount
106 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rab-7; Rab7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; ras-related protein BRL-RAS; ras-related protein p23; small GTP-binding protein; unnamed protein product
Gene Aliases: CMT2B; PRO2706; RAB7; Rab7a
UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527
Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448
autophagosome assembly
protein targeting to lysosome
endocytosis
epidermal growth factor catabolic process
endosome to lysosome transport
response to bacterium
protein transport
viral release from host cell
protein to membrane docking
retrograde transport, endosome to Golgi
cholesterol homeostasis
early endosome to late endosome transport
positive regulation of protein catabolic process
positive regulation of viral process
protein localization to lysosome
lipophagy
lysosome to ER cholesterol transport
phagosome maturation
phagosome acidification
phagosome-lysosome fusion
endosome to plasma membrane protein transport
negative regulation of exosomal secretion
positive regulation of exosomal secretion
negative regulation of intralumenal vesicle formation
synaptic vesicle recycling via endosome
bone resorption
intracellular protein transport
establishment of vesicle localization
neurotransmitter receptor transport, postsynaptic endosome to lysosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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