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Invitrogen
H2BR79me1 Recombinant Rabbit Monoclonal Antibody (ST0430)
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FIGURE: 1 / 6
H2BR79me1 Antibody (MA5-41119) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of H2BR79me1 in MCF-7 cells (green) using H2BR79me1 Recombinant Monoclonal Antibody (Product # MA5-41119). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-41119
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ST0430
Immunogen
Synthetic peptide within Human Histone H2B aa 51-100.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is predicted to work in Western Blot at a dilution of 1:1,000.
Target Information
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H2B histone family, member B; HIRA-interacting protein 2; histone 1, H2bd; histone cluster 1 H2B family member d; histone cluster 1, H2bd; Histone H2B mono methyl R79; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; histone H2B.1 B; Histone H2B.a; histone H2B.b; Histone H2B.g; Histone H2B.h; Histone H2B.k; Histone H2B.l; MONO-METHYL-HISTONE H2B (Arg79); MONO-METHYL-HISTONE H2B (R79); unnamed protein product
Gene Aliases: dJ221C16.6; H2B.1B; H2B/a; H2B/b; H2B/g; H2B/h; H2B/k; H2B/l; H2BFA; H2BFB; H2BFG; H2BFH; H2BFK; H2BFL; HIRIP2; HIST1H2BC; HIST1H2BD; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI
UniProt ID: (Human) P58876
Entrez Gene ID: (Human) 3017
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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