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beta Actin Recombinant Rabbit Monoclonal Antibody (0N2H8), HRP
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FIGURE: 1 / 2
beta Actin Antibody (MA5-42945) in WB
Western blot analysis of beta Actin in extracts of various cell lines. Samples were incubated with beta Actin Monoclonal antibody (Product # MA5-42945) using a dilution of 1:50,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42945
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0N2H8
Immunogen
Recombinant protein (or fragment)
Conjugate
HRP
HRP
HRP
Form
Liquid
Concentration
0.9 mg/mL
Amount
90 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.2% BSA
Contains
0.09% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, HepG2, MCF7, Mouse liver, Mouse kidney, Mouse lung, Rat liver, Rat kidney, Rat lung. The target is usually found in the following locations: Cytoplasm, cytoskeleton.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin, cytoplasmic 1; B-ACTZF; Bact; actin; beta actin; Beta-actin; Beta-actin-1; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin
Gene Aliases: ACTB; actba; actin; Actx; B-ACTZF; Bact; bactin1; bactzf; beta-actin; E430023M04Rik; wu:fd18f05
UniProt ID: (Pig) Q6QAQ1, (Chicken) P60706, (Rat) P60711, (Mouse) P60710, (Zebrafish) Q7ZVI7
Entrez Gene ID: (Pig) 414396, (Chicken) 396526, (Rat) 81822, (Mouse) 11461, (Zebrafish) 57934
protein binding
ATP binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
nitric-oxide synthase binding
nucleotide binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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