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Invitrogen
Hemoglobin alpha Recombinant Rabbit Monoclonal Antibody (PD00-16)
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FIGURE: 1 / 6
Hemoglobin alpha Antibody (MA5-50713) in IHC (P)
Immunohistochemistry of Hemoglobin alpha in paraffin-embedded human bone marrow tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with Hemoglobin alpha recombinant monoclonal antibody (Product # MA5-50713) with a dilution of ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-16
Immunogen
Synthetic peptide corresponding to Human Hemoglobin alpha aa 50-150.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: Rat heart tissue lysates, human bone marrow tissue, human liver tissue, human placenta tissue, mouse brain tissue, human spleen tissue.
Predicted band size: 15 kDa
Subcellular Location: Cytosol, cytosolic small ribosomal subunit, hemoglobin complex, blood microparticle, extracellular exosome, extracellular region, extracellular space, endocytic vesicle lumen, haptoglobin-hemoglobin complex, membrane.
Target Information
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-alpha globin; 2-alpha-1 globin; alpha 1 globin; alpha 2 globin; alpha globin; alpha globin 1; alpha globin chain; alpha one globin; alpha-1-globin; Alpha-1/2-globin; alpha-2 globin chain; alpha-globin; delta globin; globin c1; globin c2; globin c3; globin, alpha; haemaglobin alpha 1; hemoglobin alpha 1 chain; hemoglobin alpha 1 globin chain; hemoglobin alpha 2 chain; hemoglobin alpha chain; hemoglobin alpha, adult chain 1; hemoglobin alpha, adult chain 2; Hemoglobin alpha-1/2 chain; hemoglobin alpha-chain; Hemoglobin subunit alpha-1/2; hemoglobin, alpha 1; hemoglobin, alpha 2; major alpha-hemoglobin; unnamed protein product
Gene Aliases: ECYT7; Glnc1; Glnc2; Glnc3; GloA; Hba; Hba-a1; Hba-a2; HBA-T3; Hba1; Hba2; HBAM; Hbat1; HBH; METHBA
UniProt ID: (Human) P69905, (Rat) P01946
Entrez Gene ID: (Human) 3039, (Mouse) 15122, (Rat) 25632, (Mouse) 110257, (Rat) 360504, (Rat) 287167
in utero embryonic development
response to bacterium
carbon dioxide transport
oxygen transport
response to stilbenoid
erythrocyte development
cellular oxidant detoxification
inflammatory response
nitric oxide transport
response to estradiol
response to hydrogen peroxide
hydrogen peroxide catabolic process
negative regulation of blood pressure
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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