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Invitrogen
B7-H4 Recombinant Rabbit Monoclonal Antibody (PSH03-65)
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FIGURE: 1 / 8
B7-H4 Antibody (MA5-56381) in ICC/IF
Immunofluorescence analysis of B7H4 using SK-BR-3(positive) and HeLa (negative) cells. The cells were fixed 4% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton™ X-100 in PBS for 5 minutes, and blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were labeled with or without B7-H4 Recombinant Rabbit Monoclonal Antibody (PH03-65) (Product # MA5-56381) (green) at 1:1,000 dilution and beta tublin (red) at 1:100 dilution in 1% BSA in PBST overnight at 4 degrees Celsius and then with iFluor™ 488... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-56381
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH03-65
Immunogen
Synthetic peptide within human B7H4 aa 101-150/282.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.95 mg/mL
Amount
95 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Positive Control: MDA-MB-468 cell lysate, NIH:OVCAR-3 cell lysate, mouse uterus tissue lysate, rat ovary tissue lysate, SK-Br-3.
Tissue Specificity: Tissue enhanced (breast, fallopian tube, pancreas).
Subcellular Location: Cell membrane.
Sequence Similarities: 100% Mouse.
Predicted band size: 31 kDa.
Target Information
B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; co-stimulator molecule; costimulatory receptor; FLJ22418; immune costimulatory protein B7-H4; immunoglobulin superfamily; B7 family; RP11-229A19.4; T cell costimulatory molecule B7x; unnamed protein product
Gene Aliases: B7-H4; B7h.5; B7H4; B7S1; B7X; PRO1291; RGD1311204; VCTN1
UniProt ID: (Human) Q7Z7D3, (Mouse) Q7TSP5, (Rat) Q501W4
Entrez Gene ID: (Human) 79679, (Mouse) 242122, (Rat) 295322
regulation of cytokine production
regulation of T cell proliferation
negative regulation of T cell proliferation
negative regulation of apoptotic process
T cell receptor signaling pathway
negative regulation of T cell activation
response to protozoan
positive regulation of interleukin-2 production
positive regulation of T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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