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Invitrogen
GSDMD (Cleaved Gly276) Recombinant Rabbit Monoclonal Antibody (2S1G2)
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FIGURE: 1 / 2
GSDMD (Cleaved Gly276) Antibody (MA569594) in WB
Western blot analysis of GSDMD (Cleaved Gly276) in THP-1 cells. Samples were probed with GSDMD (Cleaved Gly276) recombinant monoclonal antibody (Product # MA569594) at 1:1,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST.
Product Details
MA569594
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2S1G2
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.95 mg/mL
Amount
95 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3., with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
GSDMD (Cleaved Gly276) refers to the activated form of gasdermin D generated following proteolytic cleavage at glycine 276, a key step in the execution of pyroptosis. Gasdermin D is encoded by the GSDMD gene and is synthesized as a full-length protein composed of an N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Upon activation of inflammatory caspases-most notably caspase-1 in canonical inflammasome pathways and caspase-4/5 (human) or caspase-11 (mouse) in noncanonical pathways-GSDMD is cleaved at a specific linker region near Gly276. This cleavage releases the N-terminal fragment, which oligomerizes and inserts into the plasma membrane to form pores, leading to cell swelling, membrane rupture, and the release of pro-inflammatory cytokines such as IL-1β and IL-18. Detection of cleaved GSDMD (Gly276) is widely used as a molecular marker of inflammasome activation and pyroptotic cell death in inflammation and innate immunity research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: breast cancer related protein; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; unnamed protein product
Gene Aliases: DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1
UniProt ID: (Human) P57764
Entrez Gene ID: (Human) 79792
plasma membrane repair
immune system process
inflammatory response
protein secretion
programmed cell death
positive regulation of interleukin-1 beta production
positive regulation of interleukin-1 production
positive regulation of interleukin-18 production
interleukin-18-mediated signaling pathway
defense response to bacterium
NLRP3 inflammasome complex assembly
innate immune response
ceramide biosynthetic process
pore complex assembly
positive regulation of inflammatory response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
protein homooligomerization
protein maturation
transmembrane transport
pyroptosis
interleukin-1-mediated signaling pathway
pyroptotic cell death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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