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Invitrogen
NEDD4L Recombinant Rabbit Monoclonal Antibody (25GB2910)
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FIGURE: 1 / 3
NEDD4L Antibody (MA570774) in WB
Western blot analysis of NEDD4L. Samples (HT-1080, HepG2, HeLa, H9C2, NIH/3T3) were incubated with NEDD4L monoclonal antibody (Product # MA570773) at a dilution of 1:20,000, followed by HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQECL Substrate Kit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA570774
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
25GB2910
Immunogen
A synthesized peptide derived from human (920-975) NEDD4-2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.75 mg/mL
Amount
15 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
Target Information
NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternatively spliced; amino-terminal variant; developmentally down-regulated 4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; KIAA0439; neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down-regulated gene 4b; similar to NEDD4; ubiquitin-ligase; neural precursor cell expressed, developmentally down-regulated 4-like protein; ubiquitin-protein ligase Rsp5; unnamed protein product
Gene Aliases: 1300012C07Rik; hNEDD4-2; NEDD4-2; NEDD4.2; Nedd4b; PVNH7; RSP5
UniProt ID: (Human) Q96PU5
Entrez Gene ID: (Human) 23327, (Mouse) 83814, (Rat) 291553
regulation of membrane depolarization
ubiquitin-dependent protein catabolic process
protein monoubiquitination
neuromuscular junction development
negative regulation of sodium ion transport
protein ubiquitination
neuron projection development
receptor internalization
regulation of protein stability
receptor catabolic process
ion transmembrane transport
regulation of membrane potential
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
regulation of dendrite morphogenesis
regulation of synapse organization
regulation of membrane repolarization
protein K48-linked ubiquitination
ventricular cardiac muscle cell action potential
negative regulation of potassium ion transmembrane transport
regulation of sodium ion transmembrane transport
negative regulation of sodium ion transmembrane transport
negative regulation of potassium ion export across plasma membrane
negative regulation of sodium ion import across plasma membrane
positive regulation of dendrite extension
negative regulation of protein localization to cell surface
positive regulation of caveolin-mediated endocytosis
regulation of sodium ion transport
negative regulation of systemic arterial blood pressure
response to salt stress
positive regulation of sodium ion transport
sodium ion import across plasma membrane
regulation of postsynaptic neurotransmitter receptor internalization
regulation protein catabolic process at postsynapse
regulation of bicellular tight junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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