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Invitrogen
Tau Monoclonal Antibody (HT7)
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Tau Antibody (MN1000) in IHC (Free)
Immunohistochemistry was performed on paraformaldehyde-fixed free-floating hippocampus tissue sections from transgenic (3xTg-AD) mice that express human Tau. Tissues were blocked in 5% normal horse serum and 0.4% Triton X-100 for 60 minutes at room temperature and probed with a Tau monoclonal antibody (Product # MN1000) at a dilution of 1:1000 at 4°C overnight. Tissues were washed extensively with PBS. Dete... View More
Product Details
MN1000
Applications
Tested Dilution
Publications
Immunohistochemistry - Free Floating (IHC (Free))
Assay-dependent
Product Specifications
Species Reactivity
Bovine,
Human
Published species
C. elegans,
Fruit fly,
Human,
Mouse,
Non-human primate,
Pig,
Rat,
Rodent
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HT7
Immunogen
Purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MN1000 recognizes normal Tau from human and bovine brain and PHF-Tau. No cross reactivity with Tau from rat brain has been observed. The epitope of this antibody has been mapped on human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.
MN1000 detects Tau which has a predicted molecular weight of approximately 79 kDa.
Purity is >95% as determined by SDS-PAGE.
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau-4; Tau5
Gene Aliases: DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU
UniProt ID: (Bovine) P29172, (Human) P10636
Entrez Gene ID: (Bovine) 281296, (Human) 4137
positive regulation of diacylglycerol kinase activity
microtubule cytoskeleton organization
neuron migration
brain development
female pregnancy
response to nutrient
adult walking behavior
axo-dendritic transport
intrinsic apoptotic signaling pathway in response to oxidative stress
response to organic substance
regulation of autophagy
regulation of microtubule polymerization
positive regulation of microtubule polymerization
negative regulation of intracellular transport
positive regulation of axon extension
mitochondrion transport along microtubule
axon extension
generation of neurons
regulation of microtubule-based movement
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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