NuPAGE™ LDS 样品缓冲液 (4X)
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NuPAGE™ LDS 样品缓冲液 (4X)
引用和文献 (9)
Invitrogen™

NuPAGE™ LDS 样品缓冲液 (4X)

NuPAGE LDS 上样缓冲液 (4X) 可针对采用 Bis-Tri 或 Tris-乙酸凝胶进行的变性凝胶电泳应用制备蛋白样品。其中含有十二烷基磺酸锂 (pH了解更多信息
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货号数量
NP000710mL
NP0008250mL
货号 NP0007
价格（CNY)
131.00
飞享价
Ends: 31-Dec-2025
193.00
共减 62.00 (32%)
Each
添加至购物车
数量:
10mL
价格（CNY)
131.00
飞享价
Ends: 31-Dec-2025
193.00
共减 62.00 (32%)
Each
添加至购物车
NuPAGE LDS 上样缓冲液 (4X) 可针对采用 Bis-Tri 或 Tris-乙酸凝胶进行的变性凝胶电泳应用制备蛋白样品。其中含有十二烷基磺酸锂 (pH 8.4)，可最大限度提高还原剂活性。

查看 SDS-PAGE 可用的所有缓冲液和试剂

NuPAGE LDS 上样缓冲液所含示踪剂染料为考马斯 G250 和酚红，而非溴酚蓝。考马斯 G250 在 MES 和 MOPS SDS 电泳缓冲液中均可显示出清晰的染料前沿，并且比溴酚蓝更靠近移动的离子迁移前沿。采用 MES SDS 电泳缓冲液时，溴酚蓝的迁移速度比部分肽段更慢。这可确保小尺寸肽段不会在凝胶上迁移。

使用方法：将 1X 稀释的样品（还原或未还原）在 70°C 下加热 10 分钟以获得理想结果。

注：NuPAGE LDS 上样缓冲液应在使用前恢复到室温 (25°C)。其未高粘度、浓缩型溶液，含有相当于常用样品缓冲液两倍的 LDS 含量。NuPAGE LDS 样品缓冲液还含有浓度较高的甘油。
仅供科研使用。不可用于诊断程序。
规格
缓冲液上样缓冲液
最大浓度4 X
凝胶兼容性NuPAGE™ 凝胶
产品线NuPAGE
产品类型LDS 样品缓冲液
数量10mL
运输条件经批准可在室温下或置于湿冰上运输
凝胶类型NuPAGE 凝胶
Unit SizeEach
内容与储存
样品缓冲液 (4X) 含有十二烷基硫酸锂 (LDS, pH 8.5)、SERVA Blue G250和酚红。

4°C 至 25°C 储存。

常见问题解答 (FAQ)

我已经使用NuPAGE LDS样品缓冲液制备了蛋白质样品。我能否煮沸样品？

可以煮沸蛋白质样品，因为煮沸不会损害缓冲液中的任何成分。我们之所以推荐将样品在70℃加热10分钟，而非煮沸样品，是因为较低的温度可减少蛋白质水解。

为什么NuPAGE样品缓冲液中使用LDS，而不是SDS？

NuPAGE 4X样品缓冲液的pH比普通Laemmli样品缓冲液高很多，它们的pH分别为8.5和6.8。在pH 8.5时，加入4X缓冲液所需浓度的SDS，会产生沉淀。此外，LDS（十二烷磺酸锂）更稳定，用于变性同样有效。

NuPAGE缓冲液应如何保存？

NuPAGE缓冲液应保存在4–25℃。

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all NuPAGE™ LDS 样品缓冲液 (4X) FAQs

引用和文献 (9)

引用和文献
Abstract
Characterization of four murine homologs of the human ov-serpin monocyte neutrophil elastase inhibitor MNEI (SERPINB1).
Authors: Benarafa Charaf; Cooley Jessica; Zeng Weilan; Bird Phillip I; Remold-O'Donnell Eileen;
Journal:J Biol Chem
PubMed ID:12189154
'The human ov-serpin monocyte neutrophil elastase inhibitor (MNEI) is encoded by a single gene SERPINB1. It is a highly efficient inhibitor of neutrophil granule proteases. Four murine genes with high sequence identity with MNEI were identified and fully sequenced, and these were named EIA, EIB, EIC, and EID. EIA, EIB ... More
Novel Modulation of Neuronal Nicotinic Acetylcholine Receptors by Association with the Endogenous Prototoxin lynx1.
Authors: Ibañez-Tallon Inés; Miwa Julie M; Wang Hai Long; Adams Niels C; Crabtree Gregg W; Sine Steven M; Heintz Nathaniel;
Journal:Neuron
PubMed ID:11906696
We previously identified lynx1 as a neuronal membrane molecule related to snake alpha-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) ... More
Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease.
Authors:Zheng L, Nukuna B, Brennan ML, Sun M, Goormastic M, Settle M, Schmitt D, Fu X, Thomson L, Fox PL, Ischiropoulos H, Smith JD, Kinter M, Hazen SL,
Journal:J Clin Invest
PubMed ID:15314690
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO(2)Tyr) and myeloperoxidase (MPO), a protein that catalyzes generation of nitrating oxidants, serve as independent predictors of atherosclerotic risk, burden, and incident cardiac events. We now show both that apolipoprotein A-I (apoA-I), the primary protein constituent of HDL, is ... More
In vivo functional assay of a recombinant aquaporin in Pichia pastoris.
Authors:Daniels MJ, Wood MR, Yeager M,
Journal:Appl Environ Microbiol
PubMed ID:16461705
The water channel protein PvTIP3;1 (alpha-TIP) is a member of the major intrinsic protein (MIP) membrane channel family. We overexpressed this eukaryotic aquaporin in the methylotrophic yeast Pichia pastoris, and immunogold labeling of cellular cryosections showed that the protein accumulated in the plasma membrane, as well as vacuolar and other ... More
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase.
Authors:Chattopadhyay MK, Tabor CW, Tabor H,
Journal:Proc Natl Acad Sci U S A
PubMed ID:14617780
In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for the ... More
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