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Invitrogen
LC3A/LC3B Polyclonal Antibody
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FIGURE: 1 / 27
LC3A/LC3B Antibody (PA1-16931) in ICC/IF
Immunocytochemistry analysis of LC3A/LC3B in HeLa cells. Samples were incubated with LC3A/LC3B polyclonal antibody (Product # PA1-16931) using a dilution of 5 µg/mL overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Left panel shows untreated HeLa cells. Right panel shows HeLa cells that were treated with 50 µM CQ overnight. Cells were fixed for 10 minutes using 10% formalin... View More
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.jpg?time=20220826?time=20220826 "LC3A/LC3B Antibody (PA1-16931) in WB, ICC/IF")
Product Details
PA1-16931
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Bovine,
Dog,
Fish,
Human,
Mouse,
Plant,
Rat,
Zebrafish
Published species
Bison,
C. elegans,
Fish,
Guinea pig,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects both LC3A and LC3B.|Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Recommended positive controls: LC3B overexpression lysate, mouse brain whole tissue lysate (adult whole normal), human brain whole tissue lysate (adult whole normal), Neuro2a whole cell lysate, Neuro2a Chloroquine-treated/untreated cell lysate, HeLa chloroquine-treated/untreated cell lysate
This antibody is predicted to react with Bovine based on 100% sequence homology.
Target Information
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; LC3; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein
Gene Aliases: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; ATG8G; LC3; LC3A; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1BLC3; MAP1LC3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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