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          • Primary Antibodies ›
          • VEGF Antibodies

          Invitrogen

          VEGF Polyclonal Antibody

          3 Published Figures
          6 References
          View all (71) VEGF antibodies

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          Cite VEGF Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (3)
          VEGF Antibody in Western Blot (WB)
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          VEGF Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          VEGF Antibody (PA1-16948) in WB

          Western blot was performed using Anti-VEGF Polyclonal Antibody (Product # PA1-16948) and 20 and 40 kDa bands corresponding to VEGF were observed in recombinant VEGF protein. Recombinant VEGF protein (250 ng) was electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabb... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          VEGF Polyclonal Antibody

          Product Details

          PA1-16948

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine, Dog, Chicken, Horse, Guinea pig, Human, Mouse, Rat

          Published species

          Guinea pig, Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide made to an internal region of the human VEGFA protein sequence (between residues 180-232).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2288261

          Product Specific Information

          In Western blot, a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA.

          Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA.

          This antibody is not recommended for IHC applications.Suggested positive control: antigen standard for VEGFA (transient overexpression lysate).

          Target Information

          VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: L-VEGF; MGC70609; RP1-261G23.1; VAS; vascular endothelial growth factor; vascular endothelial growth factor (VEGF) precursor; vascular endothelial growth factor 164; vascular endothelial growth factor 165b; vascular endothelial growth factor 188; Vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; Vascular permeability factor; Vasculotropin; VEGF; VEGF-A; VEGF1; VEGFMGC70609; VPF; VPF ant

          View more View less

          Gene Aliases: eVEGF120; eVEGF164; VEGF; VEGF-A; VEGF164; VEGFA; VPF

          View more View less

          UniProt ID: (Bovine) P15691, (Chicken) P67964, (Horse) Q9GKR0, (Dog) Q9MYV3, (Rat) P16612, (Mouse) Q00731

          View more View less

          Entrez Gene ID: (Bovine) 281572, (Chicken) 395909, (Horse) 100033839, (Dog) 403802, (Rat) 83785, (Guinea pig) 100713483, (Mouse) 22339

          View more View less

          Function(s)
          cytokine activity growth factor activity heparin binding protein homodimerization activity vascular endothelial growth factor receptor binding chemoattractant activity vascular endothelial growth factor receptor 1 binding fibronectin binding platelet-derived growth factor receptor binding vascular endothelial growth factor receptor 2 binding receptor agonist activity receptor binding neuropilin binding identical protein binding protein heterodimerization activity extracellular matrix binding protein binding growth factor
          Process(es)
          positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation positive regulation of endothelial cell migration positive regulation of protein complex assembly tube formation positive regulation of angiogenesis positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of cell division positive regulation of focal adhesion assembly cellular response to hypoxia motor neuron migration angiogenesis response to hypoxia blood vessel maturation sprouting angiogenesis epicardial cell to mesenchymal cell transition vascular endothelial growth factor signaling pathway positive regulation of vascular permeability vascular endothelial growth factor receptor signaling pathway induction of positive chemotaxis positive regulation of mast cell chemotaxis cell migration involved in sprouting angiogenesis positive regulation of receptor internalization positive regulation of cell proliferation regulation of cell shape monocyte differentiation macrophage differentiation positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter positive regulation of positive chemotaxis positive regulation of cellular component movement positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of protein kinase D signaling negative regulation of transcription from RNA polymerase II promoter ovarian follicle development blood vessel development patterning of blood vessels in utero embryonic development kidney development vasculature development lymphangiogenesis blood vessel remodeling positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation basophil chemotaxis heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter nervous system development mesoderm development female pregnancy aging lactation cell proliferation response to cold positive regulation of signal transduction positive regulation of gene expression cell migration negative regulation of cell-cell adhesion cell differentiation hyaluronan metabolic process lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development response to estradiol response to progesterone response to vitamin A cellular response to drug lymph vessel morphogenesis VEGF-activated neuropilin signaling pathway growth wound healing T-helper 1 type immune response eye photoreceptor cell development negative regulation of programmed cell death surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process endothelial cell migration cellular response to fibroblast growth factor stimulus regulation of endothelial cell differentiation negative regulation of bone resorption mRNA stabilization lung alveolus development cell maturation blood vessel morphogenesis camera-type eye morphogenesis positive regulation of smooth muscle cell proliferation neuron development cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation response to folic acid positive regulation of protein kinase B signaling primitive erythrocyte differentiation cell chemotaxis lung vasculature development mammary gland alveolus development cardiac vascular smooth muscle cell development angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis positive regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of ERK1 and ERK2 cascade response to oxygen levels dopaminergic neuron differentiation response to dexamethasone cellular response to dexamethasone stimulus commissural neuron axon guidance positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of p38MAPK cascade negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein localization to early endosome negative regulation of extrinsic apoptotic signaling pathway multicellular organism development positive regulation of receptor activity
          It has to be done as per old AB suggested Products section.

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