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Invitrogen
Gli1 Polyclonal Antibody
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FIGURE: 1 / 3
Gli1 Antibody (PA1-22557) in ICC/IF
Immunofluorescent analysis of Gli1 in 293T cells transiently transfected with Gli-1 (murine) using a Gli1 polyclonal antibody (Product # PA1-22557) at a dilution of 1:400 (red), followed by detection using a fluorescent labeled anti-rabbit IgG secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-22557
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal residues 803-818 of mouse Gli1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
PA1-22557 detects Gli1 in human and mouse samples. This antibody does not cross react with Gli2 or Gli3.
PA1-22557 has been successfully used in ELISA, immunohistochemistry (paraffin), and Western blot procedures. It is suggested that antigen retrieval should be performed using heat treatment prior to staining.
The PA1-22557 immunogen is a synthetic peptide corresponding to the C-terminal residues 803-818 of mouse Gli1.
Store at 4°C. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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