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Invitrogen
Collagen I Polyclonal Antibody
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FIGURE: 1 / 20
Collagen I Antibody (PA1-26204) in IHC (P)
Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded normal kidney using Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:100 for 4 hours at room temperature. A peroxidase goat anti-rabbit was used as a secondary antibody at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Stain... View More
Product Details
PA1-26204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Collagen Type I from human and bovine placenta.
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 8.0
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.
Target Information
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 (I) chain propeptide; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 collagen type 1; alpha-1 collagen type I; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; COL1A1; collagen 1 alpha 2 chain; collagen 1 pro-alpha-2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen Col1-ColIII-1; collagen Col1-ColIII-2; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; epididymis secretory sperm binding protein; osteogenesis imperfecta; pre-pro-alpha-2 type I collagen; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen; unnamed protein product
Gene Aliases: AA960264; AI325291; CAFYD; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSARTH1; EDSARTH2; EDSC; EDSCV; I79_010913; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim
protease binding
extracellular matrix structural constituent
protein binding
extracellular matrix structural constituent conferring tensile strength
identical protein binding
metal ion binding
platelet-derived growth factor binding
protein binding, bridging
SMAD binding
molecular_function
extracellular matrix structural protein
skeletal system development
ossification
blood vessel development
osteoblast differentiation
intramembranous ossification
endochondral ossification
response to nutrient
visual perception
sensory perception of sound
response to xenobiotic stimulus
response to mechanical stimulus
positive regulation of epithelial to mesenchymal transition
negative regulation of cell-substrate adhesion
protein transport
collagen fibril organization
positive regulation of cell migration
response to nutrient levels
response to estradiol
response to insulin
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
collagen-activated tyrosine kinase receptor signaling pathway
response to hydrogen peroxide
response to peptide hormone
skin development
skin morphogenesis
cellular response to fibroblast growth factor stimulus
positive regulation of transcription, DNA-templated
animal organ development
response to steroid hormone
skeletal system morphogenesis
embryonic skeletal system development
system development
response to cAMP
response to hyperoxia
face morphogenesis
bone trabecula formation
cartilage development involved in endochondral bone morphogenesis
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
response to fluoride
cellular response to fluoride
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
regulation of blood pressure
bone mineralization
collagen metabolic process
odontogenesis
protein heterotrimerization
extracellular matrix assembly
response to corticosteroid
wound healing
response to drug
tooth eruption
cellular response to organic substance
response to norepinephrine
cellular response to thyroid hormone stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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