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Invitrogen
Angiopoietin 1 Polyclonal Antibody
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FIGURE: 1 / 1
Angiopoietin 1 Antibody (PA5-95410) in WB
Western blot analysis of Angiopoietin 1 in Lane 1: recombinant human ANGPT1 protein (1 ng) After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Angiopoietin 1 polyclonal antibody (Product # PA5-95410) using a 0.5 µg/mL dilution, followed by a ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95410
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1 (204-241aa KHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRAT).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-1; Angiopoietin-1; KIAA0003; ligand for the TIE2 receptor; unnamed protein product
Gene Aliases: AGP1; AGPT; AGPT-1; ANG1; ANGPT1; HAE5; KIAA0003
Entrez Gene ID: (Human) 284
angiogenesis
blood vessel development
regulation of endothelial cell proliferation
sprouting angiogenesis
positive regulation of receptor internalization
negative regulation of cell adhesion
activation of transmembrane receptor protein tyrosine kinase activity
blood coagulation
positive regulation of endothelial cell migration
positive regulation of gene expression
regulation of skeletal muscle satellite cell proliferation
heparin biosynthetic process
positive regulation of protein ubiquitination
protein localization to cell surface
negative regulation of apoptotic process
negative regulation of vascular permeability
positive regulation of blood vessel endothelial cell migration
Tie signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of coagulation
positive chemotaxis
positive regulation of multicellular organismal process
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
glomerulus vasculature development
negative regulation of vascular endothelial growth factor signaling pathway
positive regulation of maintenance of permeability of blood-brain barrier
negative regulation of endothelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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