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Invitrogen
SQRDL Polyclonal Antibody
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FIGURE: 1 / 6
SQRDL Antibody (PA5-100005) in ICC/IF
Immunofluorescent analysis of SQRDL in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SQRDL polyclonal antibody (Product # PA5-100005) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SQOR(Accession Q9Y6N5), corresponding to amino acid residues V349-P399.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SQRDL.
Target Information
SQRDL (Sulfide: quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone. Although the SQRDL precursor protein displays a molecular weight of 50 kDa, a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria. SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: flavo-binding protein; liver regeneration-related protein LRRG169; sulfide dehydrogenase like; sulfide quinone reductase-like; unnamed protein product
Gene Aliases: 0610039J17Rik; 4930557M22Rik; Cc2-17; CGI-44; PRO1975; SQR; SQRDL
UniProt ID: (Human) Q9Y6N5, (Mouse) Q9R112
Entrez Gene ID: (Human) 58472, (Rat) 691966, (Mouse) 59010
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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