Invitrogen

SLC25A46 Polyclonal Antibody

查看其他7个SLC25A46抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (5)

SLC25A46 Antibody in Immunocytochemistry (ICC/IF)

SLC25A46 Antibody in Immunocytochemistry (ICC/IF)

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SLC25A46 Antibody (PA5-100184) in ICC/IF

Immunofluorescent analysis of SLC25A46 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SLC25A46 polyclonal antibody (Product # PA5-100184) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

SLC25A46 Antibody in Immunocytochemistry (ICC/IF)

SLC25A46 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SLC25A46 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SLC25A46 Antibody in Western Blot (WB)

SLC25A46 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SLC25A46 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SLC25A46 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SLC25A46 polyclonal antibody (Product # PA5-100184) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

SLC25A46 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SLC25A46 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SLC25A46 polyclonal antibody (Product # PA5-100184) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

SLC25A46 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded SLC25A46 in human colon cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with SLC25A46 polyclonal antibody (Product # PA5-100184) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

SLC25A46 Antibody in Western Blot (WB) — Western blot analysis of SLC25A46 in rat brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with SLC25A46 polyclonal antibody (Product # PA5-100184).

SLC25A46 Antibody in Western Blot (WB) — Western blot analysis of SLC25A46 in mouse brain tissue. Samples were incubated with SLC25A46 polyclonal antibody (Product # PA5-100184).

Immunogen image

SLC25A46 Polyclonal Antibody

Immunogen Image SLC25A46 Polyclonal Antibody

产品信息

PA5-100184

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SLC25A46(Accession Q96AG3), corresponding to amino acid residues G26-P76.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SLC25A46.

靶标信息

SLC25A46 belongs to the SLC25 family of mitochondrial carrier proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Mitochondrial outer membrane protein SLC25A46; Solute carrier family 25 member 46

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基因别名: 1200007B05Rik; AI325987; RGD1305072; Slc25a46

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UniProt ID: (Mouse) Q9CQS4, (Rat) Q5EB62

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Entrez Gene ID: (Mouse) 67453, (Rat) 291709

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molecular_function

transport biological_process