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Invitrogen
NOP58 Polyclonal Antibody
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FIGURE: 1 / 3
NOP58 Antibody (PA5-100645) in ICC/IF
Immunocytochemistry analysis of NOP58 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOP58 polyclonal antibody (Product # PA5-100645) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100645
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NOP58(Accession Q9Y2X3), corresponding to amino acid residues T409-A459.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NOP58.
Target Information
Nop5p, or Nop58p, is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: box C/D snoRNP protein; MSSP; similar to SIK1; 53.1 kDa; NOP58 ribonucleoprotein homolog; Nopp140 associated protein; nopp140-associated protein of 65 kDa; nucleolar protein 5; nucleolar protein NOP5/NOP58; nucleolar protein NOP58; SIK similar protein; unnamed protein product
Gene Aliases: HSPC120; MSSP; Nap65; Nol5; NOP5; NOP5/NOP58; SIK
UniProt ID: (Human) Q9Y2X3, (Mouse) Q6DFW4, (Rat) Q9QZ86
Entrez Gene ID: (Human) 51602, (Mouse) 55989, (Rat) 60373
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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