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Invitrogen
SETDB2 Polyclonal Antibody
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FIGURE: 1 / 2
SETDB2 Antibody (PA5-100857) in ICC/IF
Immunocytochemistry analysis of SETDB2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SETDB2 polyclonal antibody (Product # PA5-100857) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SETDB2(Accession Q96T68), corresponding to amino acid residues M1-Q33.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SETDB2.
Target Information
Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bifurcated 2; chronic lymphocytic leukemia deletion region gene 8 protein; CLLL8 protein; DKFZp586I0123; DKFZp761J1217; isoform lacks exon 4; lysine N-methyltransferase 1F; SET domain; SET domain bifurcated 2; similar to Setdb2 variant A but lacks 47 bps within exon 1; similar to Setdb2 variant A but utilizes an alternate splice acceptor site between exons 11 and 12, which results in a frame shift mutation and a premature stop codon; unnamed protein product; variant starts in exon 2
Gene Aliases: C13orf4; CLLD8; CLLL8; Gm293; KMT1F
UniProt ID: (Human) Q96T68
Entrez Gene ID: (Human) 83852, (Mouse) 239122
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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