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Invitrogen
CUGBP1 Polyclonal Antibody
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FIGURE: 1 / 2
CUGBP1 Antibody (PA5-101227) in IHC (P)
Immunohistochemistry analysis of CUGBP1 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CUGBP1 polyclonal antibody (Product # PA5-101227) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101227
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CELF1(Accession Q92879), corresponding to amino acid residues A75-R125.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CELF-1.
Target Information
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG) n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG) 8 oligonucleotides in vitro. CUG-BP1 is the major (CUG) 8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3-UTR. The (CUG) n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 50 kDa nuclear polyadenylated RNA-binding protein; brain protein F41; bruno-like 2; bruno-like protein 2; CELF-1; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG-BP- and ETR-3-like factor 1; CUG-BP1; CUGBP Ela; deadenylation factor CUG-BP; deadenylation factor EDEN-BP; EDEN-BP homolog; embryo deadenylation element binding protein; embryo deadenylation element-binding protein homolog; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein; RNA-binding protein BRUNOL-2; RP23-147D3.7; unnamed protein product
Gene Aliases: 1600010O03Rik; BRUNOL2; CUG-BP; CUG-BP1; CUGBP; CUGBP1; D2Wsu101e; EDEN-BP; HNAB50; NAB50; NAPOR
UniProt ID: (Human) Q92879, (Rat) Q4QQT3, (Mouse) P28659
Entrez Gene ID: (Human) 10658, (Rat) 362160, (Mouse) 13046
regulation of alternative mRNA splicing, via spliceosome
mRNA splice site selection
mRNA processing
germ cell development
negative regulation of cell proliferation
embryo development ending in birth or egg hatching
positive regulation of gene expression
negative regulation of gene expression
posttranscriptional gene silencing
negative regulation of translation
posttranscriptional gene silencing by RNA
regulation of RNA splicing
regulation of inflammatory response
mRNA destabilization
spermatid development
cerebral cortex neuron differentiation
cerebral cortex development
positive regulation of multicellular organism growth
negative regulation of epithelial cell proliferation
regulation of cell cycle
cellular response to polyamine macromolecule
gene expression
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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