Invitrogen

AARSD1 Polyclonal Antibody

查看其他8个AARSD1抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (2)

AARSD1 Antibody in Immunocytochemistry (ICC/IF)

AARSD1 Antibody in Immunocytochemistry (ICC/IF)

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AARSD1 Antibody (PA5-101410) in ICC/IF

Immunocytochemistry analysis of AARSD1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AARSD1 polyclonal antibody (Product # PA5-101410) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

AARSD1 Antibody in Immunocytochemistry (ICC/IF)

AARSD1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

AARSD1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of AARSD1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AARSD1 polyclonal antibody (Product # PA5-101410) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

AARSD1 Antibody in Western Blot (WB) — Western blot analysis of AARSD1 in extracts from various samples. Samples were incubated with AARSD1 polyclonal antibody (Product # PA5-101410). Lane 1: Hela cells (H2O2 treatment), blocked with antigen-specific peptides; Lane 2: Hela cells (H2O2 treatment); Lane 3: Mouse brain; Lane 4: 293 cells; Lane 5: Rat liver.

Immunogen image

AARSD1 Polyclonal Antibody

Immunogen Image AARSD1 Polyclonal Antibody

产品信息

PA5-101410

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human AARSD1(Accession Q9BTE6), corresponding to amino acid residues S145-P195.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total AARSD1.

靶标信息

AARSD1 functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Alanyl-tRNA deacylase alaX; Alanyl-tRNA editing protein Aarsd1; Alanyl-tRNA synthetase domain-containing protein 1; AlaX; MGC2744

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基因别名: 1110069E20Rik; 2310044P18Rik; AA589600; AARSD1; AlaX; AlaXp-II

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UniProt ID: (Rat) Q5XI97, (Mouse) Q3THG9

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Entrez Gene ID: (Rat) 619440, (Mouse) 69684

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aminoacyl-tRNA editing activity Ser-tRNA(Ala) hydrolase activity nucleic acid binding alanine-tRNA ligase activity ATP binding metal ion binding nucleotide binding protein binding ligase activity, forming aminoacyl-tRNA and related compounds RNA metabolism protein

alanyl-tRNA aminoacylation regulation of translational fidelity translation tRNA aminoacylation