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Rhodopsin Polyclonal Antibody
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FIGURE: 1 / 6
Rhodopsin Antibody (PA5-102150) in ICC/IF
Immunocytochemistry analysis of Rhodopsin in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Rhodopsin polyclonal antibody (Product # PA5-102150) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated g... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RHO(Accession P08100), corresponding to amino acid residues C323-S343.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RHO.
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4
Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Rat) P51489, (Mouse) P15409
Entrez Gene ID: (Human) 6010, (Rat) 24717, (Mouse) 212541
G-protein coupled receptor signaling pathway
visual perception
phototransduction
phototransduction, visible light
absorption of visible light
rhodopsin mediated signaling pathway
cellular response to light stimulus
transducin-mediated opsin signaling pathway
protein phosphorylation
response to light stimulus
detection of light stimulus
red, far-red light phototransduction
protein-chromophore linkage
sensory perception of light stimulus
retina development in camera-type eye
signal transduction
photoreceptor cell maintenance
response to stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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