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Invitrogen
HLA-DQA1 Polyclonal Antibody
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FIGURE: 1 / 2
HLA-DQA1 Antibody (PA5-102595) in IHC (P)
Immunohistochemistry analysis of HLA-DQA1 in rat colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HLA-DQA1 polyclonal antibody (Product # PA5-102595) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-102595
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HLA-DQA1(Accession P01909), corresponding to amino acid residues V31-P81.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HLA-DQA1.
Target Information
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DC-1 alpha chain; DC-alpha; DQ alpha; HLA class II histocompatibility antigen DQ alpha chain; HLA class II histocompatibility antigen, DQ alpha 1 chain; HLA DQ-alpha; HLA-DCA; HLA-DQ alpha chain; HLA-DQA1; HLA-DQA2; human leukocyte antigen DQA1/DRA; MHC class II antigen DQA1; MHC class II DQ alpha chain; MHC class II DQ-alpha protein; MHC class II DQA1; MHC class II HLA-D alpha glycoprotein; MHC class II HLA-DQ-alpha-1; MHC class II protein; MHC DQ alpha protein precursor; MHC HLA-DQ alpha; MHC HLA-DQ-alpha chain precursor old gene name 'HLA-DQA'; MHC HLA-DQ-alpha precursor; MHC HLA-DQA1-alpha protein; unnamed protein product
Gene Aliases: CELIAC1; DQ-A1; DQA1; HLA-DQA; HLA-DQA1; HLA-DQA1*; HLA-DQB1
UniProt ID: (Human) P01909
Entrez Gene ID: (Human) 3117
adaptive immune response
immune system process
peptide antigen assembly with MHC class II protein complex
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of immune response
positive regulation of T cell activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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