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Invitrogen
IRG1 Polyclonal Antibody
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FIGURE: 1 / 4
IRG1 Antibody (PA5-102893) in ICC/IF
Immunofluorescent analysis of IRG1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and IRG1 polyclonal antibody (Product # PA5-102893) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-102893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACOD1(Accession A6NK06), corresponding to amino acid residues I439-S481.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total IRG1.
Target Information
Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)- mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; IRG1- mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cis-aconitic acid decarboxylase; immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog
Gene Aliases: CAD; IRG1
UniProt ID: (Human) A6NK06
Entrez Gene ID: (Human) 730249
positive regulation of antimicrobial humoral response
defense response
embryo implantation
negative regulation of type I interferon production
negative regulation of toll-like receptor 2 signaling pathway
negative regulation of toll-like receptor 4 signaling pathway
cellular response to interferon-beta
negative regulation of innate immune response
negative regulation of inflammatory response
defense response to virus
cellular response to molecule of bacterial origin
cellular response to lipopolysaccharide
cellular response to cocaine
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to progesterone stimulus
tolerance induction to lipopolysaccharide
positive regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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