Search
Invitrogen
Phospho-CK1 alpha (Tyr294) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
Phospho-CK1 alpha (Tyr294) Antibody (PA5-103266) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-CK1 alpha (Tyr294) in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-CK1 alpha (Tyr294) polyclonal antibody (Product # PA5-103266) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-103266
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CSNK1A1(Accession P48729), corresponding to amino acid residues around phosphorylated Tyr294.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Casein Kinase 1 alpha only when phosphorylated at PhoTyr294.
Target Information
CNSK1A1 (CK1 alpha 1) is a member of the casein kinase I (CKI) gene family, which encodes serine/threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric, ranging from 25 to 55 kD, and are found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: casein kinase I; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; clock regulator kinase; CSNK1A1; CSNK1A1/CK1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; unnamed protein product; YIL035C
Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1; CK1a; CKIa; Csnk1a; HEL-S-77p; HLCDGP1; PRO2975
UniProt ID: (Human) P48729, (Rat) P97633, (Mouse) Q8BK63
Entrez Gene ID: (Human) 1452, (Rat) 113927, (Mouse) 93687
cell morphogenesis
protein phosphorylation
Golgi organization
signal transduction
cellular response to nutrient
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of Rho protein signal transduction
intermediate filament cytoskeleton organization
negative regulation of canonical Wnt signaling pathway
negative regulation of NLRP3 inflammasome complex assembly
positive regulation of TORC1 signaling
autophagosome assembly
negative regulation of autophagy
positive regulation of autophagy
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular response to nutrient levels
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
NLRP3 inflammasome complex assembly
positive regulation of NLRP3 inflammasome complex assembly
negative regulation of TORC1 signaling
cell surface receptor signaling pathway
Wnt signaling pathway
viral protein processing
regulation of Wnt signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support