Invitrogen

SP5 Polyclonal Antibody

Name: SP5 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-103505
Availability: InStock

查看其他5个SP5抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)

SP5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

SP5 Antibody (PA5-103505) in ICC/IF

Immunocytochemistry analysis of SP5 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SP5 polyclonal antibody (Product # PA5-103505) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image SP5 Polyclonal Antibody

产品信息

PA5-103505

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SP5(Accession Q6BEB4), corresponding to amino acid residues T260-T310.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2852841

产品详细信息

Antibody detects endogenous levels of total SP5.

靶标信息

SP5 binds to GC boxes promoters elements. SP5 probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo.

仅用于科研。不用于诊断过程。未经明确授权不得转售。