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Invitrogen
TGF alpha Polyclonal Antibody
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FIGURE: 1 / 4
TGF alpha Antibody (PA5-104226) in IHC (P)
Immunohistochemistry analysis of TGF alpha in human pancreatic cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with TGF alpha polyclonal antibody (Product # PA5-104226) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104226
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TGFA(Accession P01135), corresponding to amino acid residues V119-V160.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TGF alpha.
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protransforming growth factor alpha; TGF a; TGF α; TGF-alpha; TGFalpha; TGFα; Transforming growth factor; transforming growth factor-alpha; transforming growth factor-alpha precursor old gene name 'LU0044C'; unnamed protein product; waved 1
Gene Aliases: RATTGFAA; TFGA; TGFA; TGFAA; wa-1; wa1
UniProt ID: (Mouse) P48030, (Rat) P01134
Entrez Gene ID: (Human) 7039, (Mouse) 21802, (Rat) 24827
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
positive regulation of cell proliferation
intracellular signal transduction
ERBB2-EGFR signaling pathway
positive regulation of MAPK cascade
positive regulation of mitotic nuclear division
positive regulation of epithelial cell proliferation
hepatocyte proliferation
activation of MAPK activity
angiogenesis
negative regulation of apoptotic process
positive regulation of epidermal growth factor-activated receptor activity
negative regulation of cellular process
positive regulation of cell division
mammary gland alveolus development
wound healing
response to drug
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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