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Invitrogen
Phospho-MYL2 (Ser15) Polyclonal Antibody
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FIGURE: 1 / 16
Phospho-MYL2 (Ser15) Antibody (PA5-104265) in ICC/IF
Immunofluorescent analysis of Phospho-MYL2 (Ser15) in HepG2 during mitosis. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104265
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYL2(Accession P10916), corresponding to amino acid residues around phosphorylated Ser15.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MLC2 only when phosphorylated at Ser15.
Target Information
Thus gene encodes the regulatory light chain associated with cardiac myosin beta heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cardiac myosin light chain 2; cardiac myosin light chain-2; DKFZp779C0562; MLC-2; MLC-2s/v; Myosin light chain 2, slow skeletal/ventricular muscle isoform; myosin light chain 2v; myosin light chain phosphatase; myosin light chain, phosphorylatable, cardiac ventricles; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin, light polypeptide 2, regulatory, cardiac, slow; Ventricular myosin light chain 2; ventricular myosin regulatory light chain
Gene Aliases: MLC-2; MLC-2v; MLC2; Mlc2v; MYL2; Mylpc
UniProt ID: (Rat) P08733, (Mouse) P51667
Entrez Gene ID: (Rat) 363925, (Mouse) 17906
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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