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Invitrogen
GUCY1B3 Polyclonal Antibody
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FIGURE: 1 / 2
GUCY1B3 Antibody (PA5-104526) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded GUCY1B3 in human brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with GUCY1B3 polyclonal antibody (Product # PA5-104526) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104526
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GUCY1B3(Accession Q02153), corresponding to amino acid residues M1-L51.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GCYB1.
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta 1 sGC; GCS beta1; GCS beta3; GCS-beta-1; GCS-beta-3; GCS-beta1; GCS-beta3; GCSbeta1; GCSbeta3; guanylate cyclase 1, soluble, beta 3; Guanylate cyclase soluble subunit beta-1; Guanylate cyclase soluble subunit beta-3; guanylate cyclase, soluble, beta 1; NO-sensitive guanylyl cyclase beta 1 subunit; soluble guanylate cyclase beta-1 subunit; Soluble guanylate cyclase small subunit; unnamed protein product
Gene Aliases: GC-S-beta-1; GC-SB3; GCbeta1; GUC1B3; GUCB3; GUCSB3; GUCY1B1; GUCY1B3; SGC
UniProt ID: (Rat) P20595, (Mouse) O54865
Entrez Gene ID: (Human) 2983, (Rat) 25202, (Mouse) 54195
adenylate cyclase activity
guanylate cyclase activity
protein binding
phosphorus-oxygen lyase activity
heme binding
macromolecular complex binding
cytidylate cyclase activity
Hsp90 protein binding
nitric oxide binding
GTP binding
ion binding
metal ion binding
protein heterodimerization activity
nucleotide binding
lyase activity
guanylate cyclase
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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