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Invitrogen
RNF144A Polyclonal Antibody
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FIGURE: 1 / 3
RNF144A Antibody (PA5-104534) in IHC (P)
Immunohistochemistry analysis of RNF144A in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RNF144A polyclonal antibody (Product # PA5-104534) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RNF144A(Accession P50876), corresponding to amino acid residues H151-M201.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RNF144A.
Target Information
UBCE7IP4 (Ubiquitin-conjugating enzyme 7- interacting protein 4), also known as RNF144A (RING finger protein 144A), KIAA0161 or RNF144, is a 292 amino acid single-pass membrane protein that contains one RING-type zinc finger and two IBR-type zinc fingers. Functioning as an E3 ubiquitin-protein ligase, UBCE7IP4 accepts ubiquitin (in the form of a thioester) from E2 ubiquitin-conjugating enzymes, such as UBC8 and UBCH7, and transfers that ubiquitin residue to target substrates. Via its RING finger, UBCE7IP4 may play a role in protein-DNA and protein-protein interactions throughout the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF144A; KIAA0161; probable E3 ubiquitin-protein ligase RNF144A; ring finger protein 144; RING finger protein 144A; UbcM4-interacting protein 4; ubiquitin conjugating enzyme 7 interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4; unnamed protein product
Gene Aliases: hUIP4; KIAA0161; RGD1561737; RNF144; RNF144A; UBCE7IP4; UIP4
UniProt ID: (Mouse) Q925F3
Entrez Gene ID: (Human) 9781, (Mouse) 108089, (Rat) 500636
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ubiquitin conjugating enzyme binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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