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Invitrogen
Phospho-RyR2 (Ser2814) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-RyR2 (Ser2814) Antibody (PA5-104558) in WB
Western blot analysis of Phospho-RyR2 (Ser2814) in A431 cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-RyR2 (Ser2814) polyclonal antibody (Product # PA5-104558).
Product Details
PA5-104558
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RYR2(Accession Q92736), corresponding to amino acid residues around phosphorylated Ser2814.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of RYR2 only when phosphorylated at Ser2814.
Target Information
Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; cardiac ryanodine receptor 2; cardiac-type ryanodine receptor; hRYR-2; islet-type ryanodine receptor; kidney-type ryanodine receptor; Ryanodine receptor; Ryanodine receptor 2; ryanodine receptor 2 (cardiac); RYR-2; Type 2 ryanodine receptor; unnamed protein product
Gene Aliases: ARVC2; ARVD2; RyR; RYR-2; RYR2; VACRDS; VTSIP
UniProt ID: (Human) Q92736
Entrez Gene ID: (Human) 6262, (Dog) 403615
ion channel activity
ryanodine-sensitive calcium-release channel activity
calcium channel activity
calcium ion binding
protein binding
calmodulin binding
calcium-release channel activity
enzyme binding
protein kinase binding
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
identical protein binding
suramin binding
ion channel binding
calcium-induced calcium release activity
ligand-gated ion channel
response to hypoxia
regulation of heart rate
embryonic heart tube morphogenesis
left ventricular cardiac muscle tissue morphogenesis
cardiac muscle hypertrophy
detection of calcium ion
ion transport
calcium ion transport
cellular calcium ion homeostasis
striated muscle contraction
positive regulation of heart rate
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of cardiac muscle contraction by calcium ion signaling
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to muscle activity
calcium-mediated signaling
response to caffeine
ion transmembrane transport
response to muscle stretch
release of sequestered calcium ion into cytosol
positive regulation of sequestering of calcium ion
regulation of cytosolic calcium ion concentration
establishment of localization in cell
response to redox state
transmembrane transport
regulation of cardiac muscle contraction
cardiac muscle contraction
calcium ion transport into cytosol
sarcoplasmic reticulum calcium ion transport
calcium ion transmembrane transport
cellular response to caffeine
cellular response to epinephrine stimulus
establishment of protein localization to endoplasmic reticulum
ventricular cardiac muscle cell action potential
Purkinje myocyte to ventricular cardiac muscle cell signaling
cell communication by electrical coupling involved in cardiac conduction
type B pancreatic cell apoptotic process
calcium ion transmembrane import into cytosol
positive regulation of the force of heart contraction
regulation of AV node cell action potential
regulation of SA node cell action potential
regulation of atrial cardiac muscle cell action potential
regulation of ventricular cardiac muscle cell action potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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