Search

  • Contact Us
  • Quick Order

Invitrogen

Phospho-MST1/MST2 (Thr183, Thr180) Polyclonal Antibody

1 Reference
View all (11) MST1/MST2 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
Phospho-MST1/MST2 (Thr183, Thr180) Polyclonal Antibody

Product Details

PA5-104616

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human STK4(Accession Q13043), corresponding to amino acid residues around phosphorylated Thr183.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816091

Product Specific Information

Antibody detectsAntibody detects endogenous levels of MST1 when phosphorylated at Thr183 or MST2 when phosphorylated at Thr180.

Target Information

STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1/C STK4; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2; sterile 20-like kinase 1; unnamed protein product; Yeast Sps1/Ste20-related kinase 3

View more View less

Gene Aliases: 0610042I06Rik; AI447339; AU020804; Kas-2; KRS1; KRS2; Mess1; MST; MST1; MST2; Mst3; Ste20; STK3; STK4; YSK3

View more View less

UniProt ID: (Human) Q13188, (Human) Q13043, (Rat) O54748, (Mouse) Q9JI10, (Mouse) Q9JI11

View more View less

Entrez Gene ID: (Human) 6788, (Human) 6789, (Rat) 65189, (Mouse) 56274, (Rat) 311622, (Mouse) 58231

View more View less

Function(s)
nucleotide binding magnesium ion binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity identical protein binding protein serine/threonine kinase activator activity metal ion binding protein serine kinase activity transcription regulator activator activity protein homodimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding receptor signaling protein serine/threonine kinase activity MAP kinase kinase kinase activity transferase activity, transferring phosphorus-containing groups transcription factor binding protein dimerization activity non-receptor serine/threonine protein kinase
Process(es)
neural tube formation endocardium development protein phosphorylation protein import into nucleus apoptotic process signal transduction JNK cascade central nervous system development cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression organ growth hippo signaling positive regulation of hippo signaling intracellular signal transduction positive regulation of apoptotic process regulation of MAPK cascade protein kinase B signaling positive regulation of fat cell differentiation positive regulation of JNK cascade negative regulation of organ growth epithelial cell proliferation negative regulation of epithelial cell proliferation protein stabilization protein tetramerization centrosome separation positive regulation of protein kinase B signaling canonical Wnt signaling pathway primitive hemopoiesis cell differentiation involved in embryonic placenta development regulation of cell differentiation involved in embryonic placenta development protein localization to centrosome negative regulation of canonical Wnt signaling pathway hepatocyte apoptotic process positive regulation of extrinsic apoptotic signaling pathway via death domain receptors cell morphogenesis patterning of blood vessels positive regulation of protein phosphorylation anatomical structure morphogenesis peptidyl-serine phosphorylation keratinocyte differentiation positive regulation of peptidyl-serine phosphorylation protein autophosphorylation positive regulation of hepatocyte apoptotic process positive regulation of substrate-dependent cell migration, cell attachment to substrate signal transduction by protein phosphorylation positive regulation of protein binding positive regulation of sequence-specific DNA binding transcription factor activity MAPK cascade activation of MAPKK activity regulation of mitotic cell cycle phosphorylation stress-activated protein kinase signaling cascade
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support