Search
Invitrogen
Phospho-PAK2 (Ser20) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
Phospho-PAK2 (Ser20) Antibody (PA5-104629) in ICC/IF
Immunocytochemistry analysis of Phospho-PAK2 (Ser20) in Heat-shock treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PAK2 (Ser20) polyclonal antibody (Product # PA5-104629) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104629
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAK2(Accession Q13177), corresponding to amino acid residues around phosphorylated Ser20.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PAK2 only when phosphorylated at Ser20.
Target Information
PAK2 (serine/threonine-protein kinase PAK2) is a serine/threonine kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, or proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformation change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Alpha-PAK; Beta-PAK; gamma pak; Gamma-PAK; Oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 2; p21-activated kinase 2; p58; p65-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK1; PAK3; PAK65; S6/H4 kinase; Serine/threonine-protein kinase PAK 2
Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-2; PAK2
UniProt ID: (Mouse) Q8CIN4, (Rat) Q64303
Entrez Gene ID: (Mouse) 224105, (Rat) 29432
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
protein tyrosine kinase activator activity
identical protein binding
Rac GTPase binding
receptor signaling protein serine/threonine kinase activity
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
Rho protein signal transduction
regulation of mitotic cell cycle
metabolic process
phosphorylation
cell migration
peptidyl-serine phosphorylation
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of growth
negative regulation of apoptotic process
regulation of MAPK cascade
protein autophosphorylation
positive regulation of peptidyl-tyrosine phosphorylation
dendritic spine development
positive regulation of protein tyrosine kinase activity
cellular response to organic cyclic compound
positive regulation of extrinsic apoptotic signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
mitotic cell cycle
intracellular signal transduction
regulation of apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support