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Invitrogen
Phospho-Cardiac Troponin I (Ser44) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Cardiac Troponin I (Ser44) Antibody (PA5-104835) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-Cardiac Troponin I (Ser43) in human Heart muscle tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Cardiac Troponin I (Ser43) polyclonal antibody (Product # PA5-104835) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-r... View More
Product Details
PA5-104835
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TNNI3(Accession P19429), corresponding to amino acid residues around phosphorylated Ser44.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of TNNI3 only when phosphorylated at Serine 44.
Target Information
Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); MGC116817; sarcomeric protein; TNNI3; Tpn1; troponin 1, type 3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; unnamed protein product
Gene Aliases: CMD1FF; CMD2A; CMH7; Ctni; RCM1; Tn1; TnI; TNNC1; TNNI3
UniProt ID: (Human) P19429, (Rat) P23693, (Mouse) P48787
Entrez Gene ID: (Human) 7137, (Rat) 29248, (Mouse) 21954
vasculogenesis
regulation of systemic arterial blood pressure by ischemic conditions
skeletal muscle contraction
cellular calcium ion homeostasis
regulation of muscle contraction
regulation of smooth muscle contraction
striated muscle contraction
heart development
regulation of cardiac muscle contraction by calcium ion signaling
muscle filament sliding
negative regulation of ATPase activity
ventricular cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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