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Invitrogen
Phospho-PAK1 (Thr212) Polyclonal Antibody
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FIGURE: 1 / 6
Phospho-PAK1 (Thr212) Antibody (PA5-104983) in ICC/IF
Immunocytochemistry analysis of Phospho-PAK1 (Thr212) in H2O2 treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PAK1 (Thr212) polyclonal antibody (Product # PA5-104983) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (R... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104983
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAK1(Accession Q13153), corresponding to amino acid residues around phosphorylated Thr212.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PAK1 only when phosphorylated at Threonine 212.
Target Information
PAK1 is a serine/threonine kinase that is a target for restenosis, cancer, and immunosuppression treatment. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activated protein kinase alpha; ADRB2; alpha pak; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK-1; PAK1B; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1
Gene Aliases: AW045634; PAK-1; PAK1; Paka
UniProt ID: (Rat) P35465
Entrez Gene ID: (Mouse) 18479, (Rat) 29431
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
collagen binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
Rac GTPase binding
MAP kinase kinase kinase activity
response to hypoxia
positive regulation of protein phosphorylation
protein phosphorylation
exocytosis
apoptotic process
Rho protein signal transduction
regulation of mitotic cell cycle
neuromuscular junction development
metabolic process
response to organic substance
regulation of gene expression
phosphorylation
dendrite development
cell migration
amygdala development
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
actin cytoskeleton reorganization
activation of protein kinase activity
cellular response to insulin stimulus
regulation of actin cytoskeleton organization
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
wound healing
regulation of apoptotic process
receptor clustering
regulation of MAPK cascade
positive regulation of JUN kinase activity
protein autophosphorylation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
dendritic spine development
gamma-aminobutyric acid secretion, neurotransmission
glutamate secretion, neurotransmission
cellular response to organic cyclic compound
observational learning
regulation of long-term synaptic potentiation
MAPK cascade
activation of MAPKK activity
mitotic cell cycle
small GTPase mediated signal transduction
axonogenesis
intracellular signal transduction
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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