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Invitrogen
Phospho-CAMKIV (Thr200) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-CAMKIV (Thr200) Antibody (PA5-105011) in ICC/IF
Immunocytochemistry analysis of Phospho-CAMKIV (Thr200) in Hela cells (20 min of 30 µM Forskolin treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-CAMKIV (Thr200) polyclonal antibody (Product # PA5-105011) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated... View More
Product Details
PA5-105011
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CAMK4(Accession Q16566), corresponding to amino acid residues around phosphorylated Thr200.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of CaMK4 only when phosphorylated at Threonine 200.
Target Information
CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphoryates CREB, oncoprotein 18, and SRF. It has limited tissue distribution, and has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Ca2+/calmodulin-dependent protein kinase type IV/Gr; Calcium/calmoduli; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calmodulin-dependent protein kinase IV; Calspermin; CAM kinase IV; CAM kinase- GR; CAM kinase-GR; CaMK IV; EC 2.7.11.17; KCC4; kinase CaMK4; OTTHUMP00000222818; unnamed protein product
Gene Aliases: A430110E23Rik; AI666733; CAMK; CaMK IV; CAMK-GR; CAMK4; CAMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; RATCCDPK
UniProt ID: (Human) Q16566, (Rat) P13234
Entrez Gene ID: (Human) 814, (Mouse) 12326, (Rat) 25050
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
kinase activity
transferase activity
protein serine kinase activity
protein binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
adaptive immune response
immune system process
positive regulation of myeloid dendritic cell cytokine production
protein phosphorylation
inflammatory response
signal transduction
long-term memory
regulation of T cell differentiation in thymus
intracellular signal transduction
myeloid dendritic cell differentiation
regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
nucleocytoplasmic transport
neuron-neuron synaptic transmission
phosphorylation
peptidyl-serine phosphorylation
protein autophosphorylation
positive regulation of protein export from nucleus
myeloid dendritic cell cytokine production
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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