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Phospho-PABPN1 (Ser150) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-PABPN1 (Ser150) Antibody (PA5-105091) in ICC/IF
Immunocytochemistry analysis of Phospho-PABPN1 (Ser150) in Hela cells (20 min of 30 µM Forskolin treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PABPN1 (Ser150) polyclonal antibody (Product # PA5-105091) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105091
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PABPN1(Accession Q86U42), corresponding to amino acid residues around phosphorylated Ser150.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PABP2 only when phosphorylated at Ser150.
Target Information
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: nuclear poly(A)-binding protein 1; PABII; PABP-2; poly(A) binding protein 2; poly(A) binding protein II; poly(A)-binding protein 2; poly(A)-binding protein II; polyadenylate-binding nuclear protein 1; unnamed protein product
Gene Aliases: mPABII; OPMD; PAB2; PABII; PABP-2; PABP2; Pabp3
UniProt ID: (Bovine) Q28165, (Human) Q86U42, (Mouse) Q8CCS6
Entrez Gene ID: (Bovine) 282298, (Human) 8106, (Mouse) 54196, (Rat) 116697
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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