Invitrogen

Phospho-SRF (Ser103) Polyclonal Antibody

查看其他24个SRF抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (12)

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 12

Phospho-SRF (Ser103) Antibody (PA5-105146) in IHC (P)

Immunohistochemistry analysis of Phospho-SRF (Ser103) in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-SRF (Ser103) Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in rat colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human pancreatic cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human gastric cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human pancreatic cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human esophageal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human gastric cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in human kidney cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-SRF (Ser103) in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-SRF (Ser103) Antibody in Western Blot (WB) — Western blot analysis of Phospho-SRF (Ser103) in extracts from RAW264.7 cells (serum starvation treatment). Samples were incubated with Phospho-SRF (Ser103) polyclonal antibody (Product # PA5-105146). The lane on the left was treated with blocking peptide.

Immunogen image

Phospho-SRF (Ser103) Polyclonal Antibody

Immunogen Image Phospho-SRF (Ser103) Polyclonal Antibody

产品信息

PA5-105146

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SRF(Accession P11831), corresponding to amino acid residues around phosphorylated Ser103.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of SRF only when phosphorylated at Ser103.

靶标信息

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. SFR is required for cardiac differentiation and maturation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: OTTHUMP00000039820; Serum response factor; SRF

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基因别名: AW049942; AW240594; RGD1559787; SRF

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UniProt ID: (Mouse) Q9JM73

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Entrez Gene ID: (Mouse) 20807, (Rat) 501099

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding serum response element binding chromatin DNA binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding protein dimerization activity

patterning of blood vessels in utero embryonic development mesoderm formation neuron migration trophectodermal cell differentiation heart looping morphogenesis of an epithelial sheet cell migration involved in sprouting angiogenesis leukocyte differentiation positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter actin filament organization cell-matrix adhesion multicellular organism development gastrulation heart development long-term memory negative regulation of cell proliferation associative learning response to hormone epithelial structure maintenance positive regulation of transcription via serum response element binding single organismal cell-cell adhesion hippocampus development tangential migration from the subventricular zone to the olfactory bulb actin cytoskeleton organization contractile actin filament bundle assembly regulation of cell adhesion platelet activation platelet formation negative regulation of cell migration neuron projection development regulation of water loss via skin response to cytokine megakaryocyte development dorsal aorta morphogenesis mRNA transcription from RNA polymerase II promoter stress fiber assembly skin morphogenesis positive thymic T cell selection sarcomere organization positive regulation of cell differentiation positive regulation of axon extension positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of transcription by glucose muscle cell cellular homeostasis developmental growth erythrocyte development positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of filopodium assembly cardiac myofibril assembly hematopoietic stem cell differentiation positive regulation of transcription initiation from RNA polymerase II promoter long term synaptic depression heart trabecula formation lung morphogenesis bronchus cartilage development trachea cartilage development cardiac vascular smooth muscle cell differentiation eyelid development in camera-type eye lung smooth muscle development bicellular tight junction assembly primitive streak formation epithelial cell-cell adhesion cellular senescence negative regulation of beta-amyloid clearance response to hypoxia cellular response to glucose stimulus