Search
Invitrogen
Phospho-IRS1 (Ser323) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Phospho-IRS1 (Ser323) Antibody (PA5-105165) in WB
Western blot analysis of Phospho-IRS1 (Ser318) in 293T cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-IRS1 (Ser318) polyclonal antibody (Product # PA5-105165).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105165
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IRS1(Accession P35568), corresponding to amino acid residues around phosphorylated Ser323.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IRS1 only when phosphorylated at Ser323, which site historically referenced as Ser318.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185
Gene Aliases: G972R; IRS-1; IRS1; IRS1IRM
UniProt ID: (Rat) P35570, (Mouse) P35569
Entrez Gene ID: (Rat) 25467, (Mouse) 16367
signal transducer activity
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
protein kinase binding
protein domain specific binding
SH2 domain binding
phosphatidylinositol 3-kinase binding
positive regulation of mesenchymal cell proliferation
insulin receptor signaling pathway
regulation of gene expression
positive regulation of glucose metabolic process
phosphatidylinositol 3-kinase signaling
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
protein localization to nucleus
positive regulation of phosphorylation
response to peptide hormone
protein kinase B signaling
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
insulin-like growth factor receptor signaling pathway
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
positive regulation of glucose import in response to insulin stimulus
signal transduction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support