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Invitrogen
SIAH2 Polyclonal Antibody
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FIGURE: 1 / 4
SIAH2 Antibody (PA5-105291) in IHC (P)
Immunohistochemistry analysis of SIAH2 in human colorectal cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SIAH2 polyclonal antibody (Product # PA5-105291) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SIAH2(Accession O43255), corresponding to amino acid residues F246-C296.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SIAH2.
Target Information
SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 6.3.2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 2; seven in absentia homolog 2; siah-2; unnamed protein product
Gene Aliases: hSiah2; Sinh2
UniProt ID: (Human) O43255, (Rat) Q8R4T2, (Mouse) Q06986
Entrez Gene ID: (Human) 6478, (Rat) 140593, (Mouse) 20439
ubiquitin-dependent protein catabolic process
small GTPase mediated signal transduction
protein ubiquitination
regulation of protein ubiquitination
regulation of circadian rhythm
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
amyloid fibril formation
regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway
protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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