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Invitrogen
Phospho-GLI1 (Ser1071) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-GLI1 (Ser1071) Antibody (PA5-105346) in WB
Western blot analysis of Phospho-GLI1 (Ser1071) in PC-3 (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-GLI1 (Ser1071) polyclonal antibody (Product # PA5-105346).
Product Details
PA5-105346
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GLI1(Accession P08151), corresponding to amino acid residues around phosphorylated Ser1071.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Gli1 only when phosphorylated at Ser1071.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; Glioma-associated oncogene homolog; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; unnamed protein product; zinc finger protein 5; Zinc finger protein GLI1
Gene Aliases: AV235269; GLI; GLI1; PAPA8; PPD1; Zfp-5; Zfp5
UniProt ID: (Human) P08151, (Mouse) P47806
Entrez Gene ID: (Human) 2735, (Mouse) 14632
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
protein binding
microtubule binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
nucleic acid binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription regulatory region DNA binding
DNA-binding transcription factor
osteoblast differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
smoothened signaling pathway
spermatogenesis
spermatid development
ventral midline development
positive regulation of cell proliferation
regulation of smoothened signaling pathway
response to wounding
anatomical structure morphogenesis
epidermal cell differentiation
dorsal/ventral pattern formation
proximal/distal pattern formation
regulation of gene expression
cerebellar cortex morphogenesis
pituitary gland development
cell differentiation
lung development
positive regulation of cell migration
prostate gland development
regulation of osteoblast differentiation
positive regulation of DNA replication
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract morphogenesis
notochord regression
positive regulation of cardiac muscle cell proliferation
negative regulation of canonical Wnt signaling pathway
liver regeneration
positive regulation of cell cycle G1/S phase transition
regulation of hepatocyte proliferation
transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
multicellular organism development
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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