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Invitrogen
Phospho-Caspase 2 Precursor (Ser340) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Caspase 2 Precursor (Ser340) Antibody (PA5-105353) in ICC/IF
Immunocytochemistry analysis of Phospho-Caspase 2 Precursor (Ser340) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-Caspase 2 Precursor (Ser340) polyclonal antibody (Product # PA5-105353) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105353
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CASP2(Accession P42575), corresponding to amino acid residues around phosphorylated Ser340.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Caspase 2 only when phosphorylated at Ser340.
Target Information
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP-2; Caspase-2; ICH-1 protease; ICH-1L; ICH-1L/1S; NEDD-2; Neural precursor cell expressed developmentally down-regulated protein 2; Protease ICH-1
Gene Aliases: CASP-2; CASP2; ICH-1; ICH1; NEDD-2; NEDD2
UniProt ID: (Mouse) P29594, (Rat) P55215
Entrez Gene ID: (Mouse) 12366, (Rat) 64314
luteolysis
neural retina development
proteolysis
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
brain development
aging
intrinsic apoptotic signaling pathway in response to DNA damage
programmed cell death
protein processing
ectopic germ cell programmed cell death
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cellular response to mechanical stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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