Search
Invitrogen
Phospho-LDHA (Tyr239) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 5
Phospho-LDHA (Tyr239) Antibody (PA5-105446) in ICC/IF
Immunocytochemistry analysis of Phospho-LDHA (Tyr239) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-LDHA (Tyr239) polyclonal antibody (Product # PA5-105446) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105446
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LDHA(Accession P00338), corresponding to amino acid residues around phosphorylated Tyr239.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of LDHA only when phosphorylated at Tyr239.
Target Information
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A4; lactate dehydrogenase M; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; LDHA; M-LDH; OTTHUMP00000165206; PIG19; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; unnamed protein product
Gene Aliases: CDK1; GSD11; HEL-S-133P; l7R2; Ldh1; Ldh3; LDHM; PIG19
UniProt ID: (Human) P00338, (Mouse) P06151, (Rat) P04642
Entrez Gene ID: (Human) 3939, (Mouse) 16828, (Rat) 24533, (Rat) 29634
lactate metabolic process
ATP biosynthetic process
lactate biosynthetic process from pyruvate
NAD metabolic process
sperm motility
pyruvate catabolic process
glycolytic process
substantia nigra development
response to hypoxia
liver development
skeletal muscle tissue development
response to nutrient
response to xenobiotic stimulus
response to glucose
response to hydrogen peroxide
positive regulation of apoptotic process
response to estrogen
response to cAMP
cellular response to phorbol 13-acetate 12-myristate
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support